Buffered Peptone Water concentrate [use 20g/L] 200g
15760-200g

Buffered Peptone Water 15760 

Use 20g to make L

Diluent and non-selective pre-enrichment liquid medium for microbiological examination of food.

Disclaimer - instructions and information below are no guarantee or implied guarantee - please make as per your own inhouse usage method or as required.

DESCRIPTION 

Buffered Peptone Water is a liquid medium recommended for increasing the recovery of injured Salmonella spp. from food and associated samples prior to selective enrichment and isolation. Buffered Peptone Water is used for detection or enumeration of Enterobacteriaceae within foodstuffs. Used as diluent, Buffered Peptone Water is for the enumeration of organisms.

TYPICAL FORMULA      (g/l)

Peptone 10.0 g/L

Sodium Chloride  5.0 g/L

Phosphate buffered to final pH 7.0 ± 0.2 at 25°C

METHOD PRINCIPLE

Peptone provides amino acids, nitrogen, carbon and minerals. Sodium chloride maintains the osmotic balance of the medium. Phosphates are the buffering agents.

PREPARATION 

Suspend 20.0 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shacking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 min.

TEST PROCEDURE 

Suspend the sample in Buffered Peptone Water to make dilutions as required. For pre-enrichment, add sample to Buffered Peptone Water at a ratio of 1:10 or 1:9 depending on the method being used. Incubate at 37 ± 1°C for 16-20 hours before transfer to selective enrichment media.

INTERPRETING RESULTS

Turbidity indicates microbial growth.

APPEARANCE 

Dehydrated medium: free-flowing, homogeneous, light beige. Prepared medium: clear, light amber.

STORAGE 

The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment.

Unit Qty: ea
Availability: 3 in stock